Consensus splice site code#

However, several cases of splice sites with GC-AG, GG-AG, GT-TG, GT-CG or CT-AG dinucleotides at the splice junctions were observed ( 4 – 8 ). IntroductionĪre there any non canonical splice sites at the splice junctions? Investigation of the diversity, biological consequences, and mechanisms of the canonical and noncanonical alternative splicing events will help one to identify those transcripts which are promising for using in genetic engineering and selection of stress-tolerant plants. Why are canonical and noncanonical alternative splicing events important? The RNA components of snRNPs interact with the intron and are involved in catalysis. Assembly and activity of the spliceosome occurs during transcription of the pre-mRNA. Splicing is catalyzed by the spliceosome, a large RNA-protein complex composed of five small nuclear ribonucleoproteins (snRNPs). New exons can be inserted into the introns to create new proteins without disrupting the functionality of the original gene. Importance of RNA Splicing It assists in the evolution process by forming different combinations of exons and thereby making new and improved proteins. Mechanical splicing doesn’t physically fuse two optical fibers together, rather two fibers are held butt-to-butt inside a sleeve with some mechanical mechanism. There are two types of fiber splicing – mechanical splicing and fusion splicing. What is splicing and its types?įiber splicing is the process of permanently joining two fibers together. : not relating to, part of, or sanctioned by a canon : not canonical noncanonical literary works. During a typical gene splicing event, the pre-mRNA transcribed from one gene can lead to different mature mRNA molecules that generate multiple functional proteins.Ī splice site mutation is a genetic mutation that inserts, deletes or changes a number of nucleotides in the specific site at which splicing takes place during the processing of precursor messenger RNA into mature messenger RNA.

Consensus splice site code#

Gene splicing is a post-transcriptional modification in which a single gene can code for multiple proteins. (SPLY-sing) The process by which introns, the noncoding regions of genes, are excised out of the primary messenger RNA transcript, and the exons (i.e., coding regions) are joined together to generate mature messenger RNA. Cryptic exons, microexons and recursive splice sites often require unconventional exon definition mechanisms. Non-canonical splicing events are often tissue-specific and are particularly enriched in the central nervous system, thereby increasing proteome diversity or regulating gene expression. The canonical splice sites are those originally described and most commonly found (like in ~99% of introns) and have GT at the donor site (just after the 5′ end of the cut) and AG at the acceptor site (just before the 3′ end of the cut). 9 When to discard a canonical splice in ESTs?.8 Are there mutations in the canonical splicing site?.7 Are there any non canonical splice sites at the splice junctions?.